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Protein structure and engineering - Shared screen with speaker view
Cynthia Burrows
33:24
Please type in your questions in the chat at any time.
Katie Mouzakis
41:53
Great talk, Hannah! Do these channels also work in the reverse direction (upshock) to let water and ions in? Could you also talk a bit more about the pressure sensing mechanism for channel opening?
Anirban Banerjee
41:54
Are you considering purifying these and reconstituting activity in vitro ? Beautiful work.
Cynthia Burrows
44:26
Very interesting topic, Hannah. What about the presence of ionophores or glucose?
Stephan Hacker
45:44
Beautiful work, Hannah! Thanks for the very interesting talk!
Stephan Hacker
57:52
Hello Mary! Thank you for the interesting talk. I was wondering how you quantitatively measure the degree of modification of e.g. the different lysines in MitoNEET with pyridoxal phosphate.
Cynthia Burrows
58:46
Nice work, Mary! Is oxidation of Cys to sulfenic acid playing a role during oxidative stress? RSH to RSOH...sometimes an intermediate on the way to RSSR.
Anirban Banerjee
59:14
Do you think that a subpopulation of proteins that do not have the Fe-S cluster are getting cross linked through the cysteine ?
Anirban Banerjee
01:00:20
How sensitive is MitoNEET structurally if you take the Fe-S cluster away in an in vitro experiment ?
Anirban Banerjee
01:00:52
Great work.
Stephan Hacker
01:02:45
Hello Mary! Thank you for the interesting talk. I was wondering how you quantitatively measure the degree of modification of e.g. the different lysines in MitoNEET with pyridoxal phosphate.
Maria A. Vanoni
01:03:57
Have you looked for a PLP dependent cysteine desulfhydrase activity (instead of aminotransferase)?
Alexandria Quillin
01:04:44
Great presentation Dr. Konkle!
Jennifer Heemstra
01:07:31
And huge thanks to our weekly hosts!
Mary Konkle
01:07:53
Can I also make a plug for ACS PIs at PUIs Biochemistry session at Fall ACS? Come check out the cool work done at Undergraduate Institutions.
Katie Mouzakis
01:22:49
Thank you for the beautiful talk, Danny!
Paramjit Arora
01:22:57
Very nice presentation and beautiful CryoEM, Danny. I was wondering if you or others have discovered any single chain insulin analogs which may be easier to modify that disulfide linked chains?
Stephan Hacker
01:24:02
Thanks for the great talk, Danny! Is it known, why the snail makes a peptide that binds to the human insulin receptor or is it pure coincidence that it does?
Cynthia Burrows
01:26:31
Are there any pro-drug approaches? e.g. A blocking agent that would hydrolyze off? Or, a different disulfide?
Stephan Hacker
01:26:45
Thank you. Impressive work!
Mary Konkle
01:27:46
Great talk! Thank you!
Jane Liu
01:43:01
Thank you Daniela - that was lovely!
Markandeswar Panda
01:43:03
Excellent work and well presented.
Paramjit Arora
01:43:04
Great job, Daniela. Your organic chemistry professor is proud of you that you are thinking about helices and PPIs :) Q: have you checked if isolated peptide mimics of alpha-G show any binding?
Jennifer Heemstra
01:43:07
Fantastic talk! Is there information you can take away from the mutant studies to inform the specific sites you might want to target with small molecules and what structures might make good leads?
Stephan Hacker
01:43:13
Great talk, Daniela! From the structure, do you think the allosteric pocket of B-Raf will be ligandable with small molecules?
Katie Mouzakis
01:43:16
Excellent talk, Daniela! I really loved how you involved the students in your biochemistry lab course to explore a series of mutants in the context of a course-based undergraduate research experience.
Mary Konkle
01:43:55
^Great example of CUREs!
Katie Berry
01:44:14
Thanks for the great talk, Daniela! I know this wasn’t the heart of your work, but are there general principles that guided the initial mutations to improve expression in E. coli, or was it created with a screen? (If you wanted to apply these ideas to other kinases or enzymes) Thanks! -Swattie ‘05
Zhihong Wang
01:44:43
Nice talk, Daniela! Is the mutation that disrupts MEK/BRAF also conserved in CRAF and ARAF?
Fera Lab
01:47:49
<3
Katie Berry
01:48:11
Thanks, Daniela - will be interesting to explore the principles that improve bacterial expression :)
Todd Gruber
01:48:18
Thanks, everyone!
Zhihong Wang
02:15:34
Thank you, Cindy!
Katie Berry
02:15:43
Thanks everyone. Great to meet and chat with many of you, and hope to see you again in future weeks!
Hamda Aleissaee
02:15:48
Thank you all.
Amanda Hargrove
02:15:48
Great talks, everyone! And thanks so much Cindy for hosting!
Jane Liu
02:15:55
Thank you Cynthia and Organizers!
Mary Konkle
02:16:01
Thanks Cindy for hosting!
Hannah Malcolm
02:16:02
Thank you
Anirban Banerjee
02:16:02
Thanks